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phrl-tk (5 ng) constitutively expressing renilla luciferase  (Promega)

 
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    Promega phrl-tk (5 ng) constitutively expressing renilla luciferase
    Phrl Tk (5 Ng) Constitutively Expressing Renilla Luciferase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phrl-tk (5 ng) constitutively expressing renilla luciferase/product/Promega
    Average 90 stars, based on 1 article reviews
    phrl-tk (5 ng) constitutively expressing renilla luciferase - by Bioz Stars, 2026-05
    90/100 stars

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    phrl-tk (5 ng) constitutively expressing renilla luciferase  (Promega)
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    Notch activation upregulates miR-218 and its host gene Slit2 in ECs (A) HUVECs were transfected with AdNIC or AdCtrl adenovirus for 48 h. Hey1, Slit2, and miR-218 RNA levels were determined by quantitative real-time PCR (n = 5). (B) HUVECs were cultured in the presence of DAPT (25 μM) or DMSO for 48 h, and Hey1, Slit2, and miR-218 RNA levels were determined by quantitative real-time PCR (n = 4). (C) HUVECs were cultured with dishes coated with Dll4 or PBS for 48 h. Hey1, Slit2, and miR-218 RNA levels were evaluated by quantitative real-time PCR (n = 3). (D) HUVECs were cultured as in (C) in the presence or absence of DAPT for 48 h. Hey1, Slit2, and miR-218 RNA levels were determined by quantitative real-time PCR (n = 4). (E) Reporter assay. A representative illustration of the structure of Slit2 gene and the reporter is shown on the left. Luciferase activity was determined and calibrated in HEK293T cells transfected with pGL3-Slit2 (100 ng), phRL-TK (5 ng), and increasing amount of pEFBOS-NIC (0, 50, 100 ng) for 48 h (n = 8). Error bars, means ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Notch activation promotes endothelial quiescence by repressing MYC expression via miR-218

    doi: 10.1016/j.omtn.2021.07.023

    Figure Lengend Snippet: Notch activation upregulates miR-218 and its host gene Slit2 in ECs (A) HUVECs were transfected with AdNIC or AdCtrl adenovirus for 48 h. Hey1, Slit2, and miR-218 RNA levels were determined by quantitative real-time PCR (n = 5). (B) HUVECs were cultured in the presence of DAPT (25 μM) or DMSO for 48 h, and Hey1, Slit2, and miR-218 RNA levels were determined by quantitative real-time PCR (n = 4). (C) HUVECs were cultured with dishes coated with Dll4 or PBS for 48 h. Hey1, Slit2, and miR-218 RNA levels were evaluated by quantitative real-time PCR (n = 3). (D) HUVECs were cultured as in (C) in the presence or absence of DAPT for 48 h. Hey1, Slit2, and miR-218 RNA levels were determined by quantitative real-time PCR (n = 4). (E) Reporter assay. A representative illustration of the structure of Slit2 gene and the reporter is shown on the left. Luciferase activity was determined and calibrated in HEK293T cells transfected with pGL3-Slit2 (100 ng), phRL-TK (5 ng), and increasing amount of pEFBOS-NIC (0, 50, 100 ng) for 48 h (n = 8). Error bars, means ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Article Snippet: In other cases, HUVECs were co-transfected with 100 ng pGL-MycUTR5, pGL-MycUTR3, pGL-hnRNPAUTR3, pGL-EYAUTR3, together with phRL-TK (5 ng) and pGV317-miR-218 (0, 50, 100 ng, Genechem).

    Techniques: Activation Assay, Transfection, Real-time Polymerase Chain Reaction, Cell Culture, Reporter Assay, Luciferase, Activity Assay